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Description

The ECM 630 is a versatile exponential decay wave electroporation system that can be used for high throughput applications. When used with the HT-200 or HT-100 Plate Handler, and BTX disposable Electroporation Plates, the ECM 630 is a powerful tool for electroporation in standard 96 well plate format or 25 well optimizer plates.

For assistance in converting cuvette protocols for use in Electroporation Plates, please contact BTX Technical Service at 1-800-272-2775 or 508-893-8999.

Applications

Modern cell biology research frequently needs the ability to transfect hundreds if not thousands of samples at once. Using traditional electroporation techniques, such as cuvettes, would be time-consuming and expensive, if not impossible. High throughput electroporation allows the user to accelerate research by conducting multiple experiments at one time on a single plate.

High Throughput Transfection and Transformation
When a large number of samples need to be transfected, minimizing the number of separate transfer steps saves time and work and therefore ultimately money. In the quest for more efficient methods, assay systems are being miniaturized to allow the handling of more samples in 96 or 25 well formats.

Advanced electroporation applications in transfection include genomic library construction, genome sequencing studies, subcloning, mutagenesis, and directed evolution studies.

High Throughput Screening for Target ID and Validation
Biomolecular screening is one of the principal tools used for the discovery of new drugs in the biopharmaceutical industry today. Batches of compounds are being tested for binding activity or biological activity against target molecules. High throughput screening seeks to screen large numbers of compounds rapidly and in parallel.

Electroporation applications in this context include antisense oligonucleotide delivery bacterial transformation with plasmid DNA, or transfection of neurons and stem cells.

Genomics and Proteomics
Bacterial transformation is a valuable way to investigate gene function in bacteria. Recent advances in genomics, the study of genes and their function, are bringing about a revolution in our understanding of the molecular mechanisms of disease, including the complex interplay of genetic and environmental factors.

Genomics is also stimulating the discovery of breakthrough healthcare products by revealing thousands of new biological targets for the development of drugs, and by giving scientists innovative ways to design new drugs, vaccines, and DNA diagnostics.

Sequencing the genome established a firm foundation-but proteomics, the study of the full set of proteins encoded by a genome, is the next, more challenging phase in the process of understanding cellular biochemistry and mechanisms of disease. High throughput electroporation will facilitate this exciting area of research.

Technical Specifications

Standard Capabilities
	Interface:	Digital User Interface
	Input:	110 V/220 V Universal
	Charge Time:	5 sec maximum
	Voltage Range:	10 - 500 V LV Mode/ 1 V resolution 50 - 2500 V HV Mode/ 5 V resolution
	Capacitance Range:	1 µF, 25 µF - 3275 µF LV Mode/25 µF resolution 25 µF, 50 µF HV Mode
	Resistance Range:	25 ohm - 1575 ohm/ 25 ohm resolution HV & LV Modes "None" setting to simulate alternative systems lacking resistance control LV Mode
	Precision Pulse™	Over 8500 RC Time Constants LV Mode 284 RC Time Constants HV Mode
	Safety:	Short Circuit Proof Maximum Pulse Delay 10 s
Other Electrical Characteristics
	Amperage:	6000 A limit LV Mode 3000 A limit HV Mode
Physical Characteristics
	Footprint:	12.5" x 12.25" x 5.5" (14 x 31.8 x 31.1 cm) (W x D x H)
	Weight:	10 lbs. (4.5 kg)
	Display:	20 x 4 character LCD
	Controls:	Single Rotary Encoder with push button toggle between all set parameters. Additional on/off Power and Start switches
Monitoring		Monitoring and display of Vpeak and RC Time Constant