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Applications

Cell biology research frequently needs the ability to transfect hundreds if not thousands of samples at once. Using traditional electroporation techniques, such as cuvettes, would be time-consuming and expensive, if not impossible. High throughput electroporation allows the user to accelerate research by conducting multiple experiments at one time on a single plate.

Advanced Transfection and Transformation
When a large number of samples need to be transfected, minimizing the number of separate transfer steps saves time and work and therefore ultimately money. In the quest for more efficient methods, assay systems are being miniaturized to allow the handling of more samples in 96 or 25 well formats.

High throughput electroporation applications in transfection include genomic library construction, delivery of siRNA, genome sequencing studies, subcloning, mutagenesis, and directed evolution studies.

High Throughput Screening for Target ID and Validation
Biomolecular screening is one of the principal tools used for the discovery of new drugs in the biopharmaceutical industry today. Batches of compounds are tested for binding activity or biological activity against target molecules. High throughput screening seeks to screen large numbers of compounds rapidly and in parallel.

Electroporation transfection applications in this context include antisense oligonucleotide delivery and siRNA delivery to cells for identification and validation of potential targets.

Genomics and Proteomics
Transfection is a valuable way to investigate gene function in cells. Recent advances in genomics, the study of genes and their function, are bringing about a revolution in our understanding of the molecular mechanisms of disease, including the complex interplay of genetic and environmental factors.

Genomics is also stimulating the discovery of breakthrough healthcare products by revealing thousands of new biological targets for the development of drugs, and by giving scientists innovative ways to design new drugs, vaccines, and DNA diagnostics.

Sequencing the genome established a firm foundation-but proteomics, the study of the full set of proteins encoded by a genome, is the next, more challenging phase in the process of understanding cellular biochemistry and mechanisms of disease. High throughput electroporation will facilitate this exciting area of research.

Technical Specifications

Standard Capabilities
	Interface:	Digital User Interface
	Input:	110 V/220 V Universal
	Charge Time:	13 sec maximum
	Voltage Range:	5 - 500 V LV Mode/ 1 V resolution 505 - 3000 V HV Mode/ 5 V resolution
	Pulse Length Range:	1 ms - 999 ms LV Mode/ 1 ms resolution 10 µsec - 999µsec LV Mode/ 1 µsec resolution 1 sec - 10 sec LV Mode/ 0.1 sec resolution 10 µs - 600 µs HV Mode/ 1 µs resolution
	Multiple Pulsing:	1 - 99 pulses
	Pulse Interval:	100 msec - 10 sec
	Programmability:	Storage for 3 setups (V, t, n, interval)
	Arc Control:	Arc Quenching

Other Electrical Characteristics
	Capacitance:	4000 µF LV, 110 µF HV
	Amperage:	500 A LV, 100 A HV

Physical Characteristics
	Footprint:	12.5" x 12.25" x 5.5" (14 x 31.8 x 31.1 cm) (W x D x H)
	Weight:	15 lbs. (6.8 kg)
	Display:	20 x 4 character LCD
	Controls:	Single Rotary Encoder with push button toggle between all set parameters. Additional on/off Power and Start switches

Monitoring:		Displays all set parameters after pulsing